Occulo employs proprietary, cutting-edge algorithms to rapidly reengineer protein therapeutics so as to reduce immunogenicity and maintain efficacy. The following trail-blazing papers from the co-founders’ university laboratories provide a sense of the fundamental principles underlying computationally-driven deimmunization, and describe extensive in vitro and in vivo validation of globally optimized biotherapeutics.

  • DisruPPI: Structure-based computational redesign algorithm for protein binding disruption, Bioinformatics 2018 in press
  • Computationally-driven identification of antibody epitopes, Elife 2018 [pubmed]
  • Computationally optimized deimmunization libraries yield highly mutated enzymes with low immunogenicity and enhanced activity, PNAS 2017 [pubmed]
  • Computationally driven antibody engineering enables simultaneous humanization and thermostabilization, Protein Eng Des Sel, 2016 [pubmed]
  • Design and engineering of deimmunized biotherapeutics, Curr Opin Struct Biol, 2016 [pubmed]
  • Antibody humanization by structure-based computational protein design, mAbs, 2016 [pubmed]
  • Depletion of T cell epitopes in lysostaphin mitigates anti-drug antibody response and enhances antibacterial efficacy in vivo, Chem Biol, 2015 [pubmed]
  • Structure-based redesign of lysostaphin yields potent anti-staphylococcal enzymes that evade immune cell surveillance, Mol Ther Methods, 2015 [pubmed]
  • Protein deimmunization via structure-based design enables efficient epitope deletion at high mutational loads, Biotechnol Bioeng, 2015 [pubmed]
  • Structure-based design of combinatorial mutagenesis libraries, Protein Sci, 2015 [pubmed]
  • Mapping the Pareto optimal design space for a functionally deimmunized biotherapeutic candidate, PLoS Comput Biol, 2015 [pubmed]
  • Computationally driven deletion of broadly distributed T cell epitopes in a biotherapeutic candidate, Cell Mol Life Sci, 2014 [pubmed]
  • A high throughput MHC II binding assay for quantitative analysis of peptide epitopes, J Vis Exp, 2014 [pubmed]
  • Structure-based redesign of proteins for minimal T-cell epitope content, J Comput Chem, 2013 [pubmed]
  • Structure-guided deimmunization of therapeutic proteins, J Comput Biol, 2013 [pubmed]
  • Design and analysis of immune-evading enzymes for ADEPT therapy, Protein Eng Des Sel, 2012 [pubmed]
  • A divide-and-conquer approach to determine the Pareto frontier for optimization of protein engineering experiments, Proteins, 2012 [pubmed]
  • Optimization of therapeutic proteins to delete T-cell epitopes while maintaining beneficial residue interactions, J Bioinform Comput Biol, 2011 [pubmed]
  • Optimization algorithms for functional deimmunization of therapeutic proteins, BMC Bioinformatics, 2010 [pubmed]